Фото: Nacho Doce / Reuters
This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.。业内人士推荐91视频作为进阶阅读
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